2022 Fiscal Year Final Research Report
Deciphering functional significance of newly generated neurons at postnatal stage investigated by multi-photon 4D imaging
Project/Area Number |
20H04122
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 59040:Nutrition science and health science-related
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Research Institution | Kyoto University |
Principal Investigator |
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | 嗅覚 / 2光子イメージング / カルシウムセンサー / 顆粒細胞 |
Outline of Final Research Achievements |
Neurogenesis occurs continuously in the mammalian postnatal brain, including humans, and newborn neurons are incorporated into the neural circuit in the olfactory bulb as granule cells. However, it is not well understood how these neurons respond to odor stimuli. In this study, we analyzed the function of granule cells using calcium imaging. First, we established an in vivo 4D imaging technique to monitor activity from different layers of the olfactory bulb. Then, we performed in vivo calcium imaging of granule cells and found that their activity was attenuated by the repeated presentation of the same odor stimulus. On the other hand, the activity of granule cells increased as learning progressed during the go/no-go olfactory discrimination task.
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Free Research Field |
神経科学
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Academic Significance and Societal Importance of the Research Achievements |
自閉症をはじめとする発達障害のメカニズムの解明および治療法の確立は最重要課題のひとつとなっているが、これら多くの病態について、生後発達期~若年成人期におけるニューロン新生低下による高次脳機能障害が示唆されている。したがって、生後発達期に産まれたニューロンの機能について、単一ニューロンレベルあるいはネットワークレベルで理解することは、ニューロン新生の基礎的理解にとどまらず、発達障害などの脳機能障害の治療法の確立し、健康・医療の質の向上ために重要であると考えられる。
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