2023 Fiscal Year Final Research Report
Identification and function analysis of sulfane sulfur-binding proteins responsible for detoxification of environmental electrophiles
| Project/Area Number |
20H04340
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 63030:Chemical substance influence on environment-related
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| Research Institution | Tokyo University of Pharmacy and Life Science (2023)
University of Tsukuba (2020-2022) |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | サルフェン硫黄 / 環境中親電子物質 / 親電子ストレス / 毒性防御 |
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| Outline of Final Research Achievements |
In this study, we aimed to isolate and identify sulfane sulfur-binding proteins from mouse organs and to clarify the protective function of these proteins. As a result, we isolated and identified growth inhibitory factor/metallothionein-3 (GIF/MT-3) as one of the sulfane sulfur-binding proteins from mouse brain cytosol. By analyzing the recombinant GIF/MT-3 protein, we found that GIF/MT-3 has the function of inactivating methylmercury, an environmental electrophile, by converting it to a sulfur adduct. We also found that GIF/MT-3 protects cells from methylmercury toxicity in cultured cell system. These results reveal a part of the intracellular molecular mechanism that play a role in protection against environmental electrophiles.
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| Free Research Field |
環境生物学
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| Academic Significance and Societal Importance of the Research Achievements |
生体内に存在する分子として近年注目を浴びているサルフェン硫黄であるが、その結合タンパク質や機能の実態については分かっていないことも多い。本課題の実施により、申請者が確立したタンパク質結合性のサルフェン硫黄を感度良く定量できる方法とカラムクロマトグラフィーを組み合わせた手法で、サルフェン硫黄結合タンパク質について解析できることを示したことから、学術的にも意義深い。また、親電子ストレス防御に働くタンパク質とそのメカニズムの一端を明らかにできたことで、健康リスクの軽減に繋がる防御戦略の構築に必要な知見が提供できた点で社会的な意義もあると言える。
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