2023 Fiscal Year Final Research Report
Transformation of lignin-derived aromatics in wood biomass into nonaromatic polymeric substances with fluorescent activities
| Project/Area Number |
20H04371
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 64030:Environmental materials and recycle technology-related
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
松藤 寛 日本大学, 生物資源科学部, 教授 (70287605)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | リグニン / 未利用バイオマス / 有機蛍光物質 / 非ベンゼン性 |
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| Outline of Final Research Achievements |
We recently reported that Pseudomonas sp. ITH-SA-1 can transform the lignin-derived aromatic compound syringaldehyde (SYAL) into non-aromatic polymeric substances with fluorescent activity (NAPSFA). We subsequently showed that the NAPSFA are produced by a non-biological process. The NAPSFA are particularly rare organic substances that fluoresce despite the absence of aromatic rings. In this study, we examined most effective productions of NAPSFA using various biomass.
When Pseudomonas sp. ITH-B52 was cultured in LB broth supplemented with syringic acid, the NAPSFA with the highest fluorescence intensity were produced. The NASPFA used for further downstream analyses. On the other hand, safety examinations reveled that the NASPFA has extremely low biological toxicity compared to conventional products. These results suggested that the NAPSSFA is a useful next-generation chemical product that is expected to have new uses.
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| Free Research Field |
環境バイオテクノロジー
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| Academic Significance and Societal Importance of the Research Achievements |
有機蛍光物質は、バイオ医薬、バイオイメージング、有機ELなど応用可能な高機能物質であり、従来型のものは基本的にその分子内にベンゼン環構造を含んでいる。これに対しわれわれは、自身の分子構造にベンゼン環を含まないNAPSFAを見い出した。当初、NAPSFAは特殊な微生物が生産するレアな物質と考えられていたが、本研究により、ベンゼン環構造を含まない蛍光物質群が化学合成できることが示され、有機蛍光物質には、ベンゼン系および非ベンゼン系の物質群が存在することが明らかとなった。また、NAPSFAは極めて安全性が高いと考えられることから、今後の応用が期待される有用な次世代化成品であると考えられる。
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