Development of dynamic and functional imaging technology for constructed 3-dimensional tissue using probes to detect cellular biological functions

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H04515
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: Osaka Dental University (2021-2022); Kyoto University (2020)
• Principal Investigator: Jo Jun-ichiro 大阪歯科大学, 歯学部, 准教授 (60511243)
• Co-Investigator(Kenkyū-buntansha): 山下 潤 東京大学, 医学部附属病院, 特任教授 (50335288)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: モレキュラービーコン / イメージング / 細胞機能 / ナノ粒子 / 3次元組織

Outline of Final Research Achievements

Construction of 3-dimensional tissue has been performed aiming at the application for regenerative medicine, disease research, and drug screening research. However, the development of evaluation technologies for constructed 3-dimensional tissues is limited and evaluation methods of tissues under living condition have not established yet. This study attempted to develop a “dynamic and functional imaging technology for constructed 3-dimensional tissue” which visualizes the dynamics and biological function of cells in constructed 3-dimensional tissue under living condition. The dynamic and functional imaging technology for constructed 3-dimensional tissue was developed based on the cell function imaging technology which was developed by the application of intracellular delivery technology for a molecular beacon to sequence-specifically detect an mRNA.

Free Research Field

バイオマテリアル

Academic Significance and Societal Importance of the Research Achievements

われわれが開発してきた細胞機能イメージング技術を基盤として構築した3次元組織の動態・機能イメージング技術は、あらゆる3次元組織構築法に適用可能であるため、基盤となる細胞機能イメージング技術の汎用性の高さも相まって、非常に汎用性の高い技術が得られる。得られた3次元組織の動態・機能イメージング技術は、構築した3次元組織の新しい非侵襲的評価手法を創出し、再生医療、疾患研究、および創薬研究に大きな進展をもたらすことは疑いない。