2021 Fiscal Year Annual Research Report
Development of novel transfection system using DNA gel
| Project/Area Number |
20J11059
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
MASUKAWA Marcos 東京工業大学, 情報理工学院, 特別研究員(DC2)
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| Project Period (FY) |
2020-04-24 – 2022-03-31
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| Keywords | DNA origami / colloidosome / self-assembly / aqueous two-phase system |
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| Outline of Annual Research Achievements |
In recent research, the interaction between self-assembled DNA structures and liquid-liquid phase-separated systems composed of polymers was investigated. It was observed that DNA structures can be selectively taken up by these compartments, leading to self-assembly that mimics artificial cells and the interaction of nucleic acids and organelles. Based on these findings, I developed a method for purifying self-assembled DNA structures and created a new category of colloidossomes based on the accumulation of DNA origami at the interface of droplets. This enabled the creation of colloidosomes that can self-organise into cellular like structures. In the future, I will explore the possibility of translocating nucleic through membranes of DNA coloidosomes, which might find application in DNA sequencing technology and on synthetic cell models for the transport of nucleic acids thourgh membranes.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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