2021 Fiscal Year Annual Research Report

Development of Novel mRNA Delivery Systems based on Flexible Biodegradable Block Copolymers

Research Project

Project/Area Number	20J13458
Research Institution	The University of Tokyo
Principal Investigator	楊文セイ東京大学, 工学系研究科, 特別研究員(DC2)
Project Period (FY)	2020-04-24 – 2022-03-31
Keywords	Nanomedicine / Polymeric micelles / messenger RNA
Outline of Annual Research Achievements	Here, we focused on the development of nanocarriers for safe and stable systemic delivery of mRNA. Thus, we have developed a library of polymers, and chemically modified with amino acids which could stable mRNA by hydrophobic interaction and π-π interaction. We confirmed that these polymers were biodegradable in physiological conditions. Polymeric micelles prepared by polymers with π-π interaction were found to be safe in vitro after incubation with 50% Fetal Bovine Serum, could achieve a highest mRNA expression in vitro. We found that micelles with π-π interaction with mRNA showed higher protein expression in the lung of mouse after 24 h intratracheal administration. Moreover, these micelles maintained over 10% of loaded mRNA in mice blood after 10 mins of intravenous administration.
Research Progress Status	令和3年度が最終年度であるため、記入しない。
Strategy for Future Research Activity	令和3年度が最終年度であるため、記入しない。