2020 Fiscal Year Annual Research Report
マイクロヒーター・ナノワイヤを用いた分子交雑に基づくDNA配列解析法の創出
| Project/Area Number |
20J15564
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| Research Institution | Nagoya University |
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Principal Investigator |
高橋 博美 名古屋大学, 工学研究科, 特別研究員(DC2)
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| Project Period (FY) |
2020-04-24 – 2022-03-31
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| Keywords | ZnO nanowires / Microfluidic |
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| Outline of Annual Research Achievements |
Detection of cell-free DNA (cfDNA) has an impact on DNA analysis in liquid biopsies. However, current strategies to detect cfDNA have limitations that should be overcome, such as having low sensitivity, and requiring much time and a specialized instrument. Here a device having a microheater integrated with zinc oxide nanowires was developed to detect target single-stranded DNAs (ssDNAs) based on DNA probe hybridization. First, it was confirmed that the microheater could constantly generate suitable heat. Second, it was confirmed that the use of SNA-MBs to specifically detect target ssDNAs. By combining the special properties of ZnO-NWs and the microheater, it was confirmed that the integrated device could detect target ssDNAs using in-situ annealed probes.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
This work has already published. (Hiromi T, et al. Microheater-integrated zinc oxide nanowire microfluidic device for hybridization-based detection of target single-stranded DNA. Nanotechnology, (2021))
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| Strategy for Future Research Activity |
After confirming that ZnO nanowire device enable to capture nucleic acid, the next step will be focused on miRNA analytical platform due to it has gained extensive attention recently for the molecular analysis. Recent technological advancements have led to the development of miRNA detection technologies: direct and nucleic acid amplification-based detection. However, there are still challenge such as low sensitivity and specificity, and time-consuming. Thus, realizing the technology for improving the collection of RNAs from biological fluids is the cornerstone to achieving RNA detection in liquid biopsies, thus enhancing the detection sensitivity. Here, the ZnO/Al2O3 core-shell nanowire device is proposed to enhance the sensitivity of miRNA analysis.
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