2021 Fiscal Year Annual Research Report
Signal-amplified two-way detection of multiple viruses using chromogen-embedded nanocarriers
| Project/Area Number |
20J22358
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| Research Institution | Shizuoka University |
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Principal Investigator |
コリス インドラ メンディ 静岡大学, 自然科学系教育部, 特別研究員(DC1)
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| Project Period (FY) |
2020-04-24 – 2023-03-31
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| Keywords | Biosensor / Virus Detection / Signal Enrichment / colorimetric / Polymer nanomaterial / Derivative spectroscopy / Analytical Chemistry / Amplified Immunoassay |
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| Outline of Annual Research Achievements |
In this year, the progress focused on nanocarrier adaption for virus sensing immunoassay. Compared to previous year, the developed chromogenic nanocarrier utilized co-precipitation in encapsulating the signal substrates within the polymer nanovesicle. The nanoprecipitation utilized the solvent variation and showcased a good encapsulation of the signal substrates and have been applied for influenza virus detection. Further, the nanomaterial was used to detect subtype Influenza Virus following the derivative of the absorbance to process two detection signals in a one-pot and simultaneous detection. Considering the result and the prior study, higher encapsulation attributed to the amplified detection signal and provided a binary signal for detection of mutating respiratory virus.
To establish a multiplex detection based on the signal, this year aims to study and investigate a binary nanocarrier in introducing various signal molecules for the virus identification. Considering the result and the prior study, higher encapsulation efficiency is the main factor to amplify the detection signal. We can achieve high encapsulation degree for the chromogens and applied a single stimulus to release two signal distinguishable chromogens in the respective nanocarrier. This year, the progress concluded the successful implementation of polymeric nanocarrier for binary virus identification based on the introduction of the substrate signal.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Following the proposal and the research plan, the progress followed the successive of the nanocarrier preparation and centralized to modulate the virus detection. Based on the research progress, this study is completed up to the preparation of high-loading chromogen in the nanocarrier in form of co-precipitation of the polymer and the chromogen. The platform could demonstrate beneficial binary response detection of respiratory viruses. This work has been disclosed and published in Journal 'ACS Applied Material and Interface (IF:9.229)’(ACS Appl. Mater. Interfaces, 13(31), 36868-36879). and communicated in domestically and internationally in symposium and conferences. This work was also awarded ‘best research award’ during the international symposium in South Korea.
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| Strategy for Future Research Activity |
The next step of this work is to establish two-way detection using signal enrichment strategy for virus sensing platform fabrication. Two-way detection requires colorimetric signal transducer and electrochemical signal generation. Reflecting from the current progress, the sensing is occurring within the solution’s area of interest, however in the electrochemical platform, the sensing involves interfacial area of interest, meaning that besides signal enrichment strategy, it required a strategy to bring the signal substrates to the interfacial of the electrode. Hence, on the last term of this fellowship, the study will aim to bring the conceptual signal enrichment strategy to the electrochemical platform with two-way detection readout, visually and electrochemically.
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