Development of a new method for quantitative analysis of alginate using complete alginate degrading enzyme

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K02344
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 08030:Family and consumer sciences, and culture and living-related
• Research Institution: Mie University
• Principal Investigator: Shibata Toshiyuki 三重大学, 生物資源学研究科, 准教授 (50380796)
• Co-Investigator(Kenkyū-buntansha): 田中 礼士 三重大学, 生物資源学研究科, 准教授 (80447862)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: アルギン酸 / アルギン酸リアーゼ / アルギン酸デオキシ糖 / DEH / 定量分析 / LC/MS

Outline of Final Research Achievements

4-Deoxy-L-erythro-5-hexoseulose uronic acid (DEH) is the only monosaccharide (alginate deoxysugar) produced from alginic acid by the action of exo-alginate lyases. The purpose of this study was to develop a new quantitative analysis method for alginic acid using DEH as a standard, which can be applied to the analysis of foods containing alginic acid. The following two research results were obtained:(1) alginate lyase was selected from the alginate-degrading and assimilating bacterial library, and the best-mixed enzyme capable of completely degrading alginate to DEH was developed, and (2) a quantitative analysis method for alginic acid was completed by testing the detection of DEH by the LC/MS method and the evaluation of analytical accuracy.

Free Research Field

食品化学

Academic Significance and Societal Importance of the Research Achievements

グリクロナンのアルギン酸は，増粘剤や安定剤として数多くの加工食品に利用されている．コレステロール吸収低減作用を持つことから，アルギン酸は，特定保健用食品の関与成分としても認可されている．これまで，アルギン酸の定量分析法は，ウロン酸への呈色反応を利用した方法が多く用いられてきたが，ペクチンをはじめ様々なマトリクスを含む加工食品への適用は，難しかった．この研究では，エキソ型アルギン酸リアーゼとDEHを用いたアルギン酸に特異的な定量分析法の開発を試みており，アルギン酸含む加工食品の品質管理に活用することが出来る．