2023 Fiscal Year Final Research Report
Development of a kit for the detection of bad S. mutans, a NASH hate factor
| Project/Area Number |
20K02352
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 08030:Family and consumer sciences, and culture and living-related
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| Research Institution | Hiroshima International University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
北川 雅恵 広島大学, 病院(歯), 助教 (10403627)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | LAMP / う蝕 / 遺伝子検査 / タブレット型培地 / ミュータンス菌 / cnm |
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| Outline of Final Research Achievements |
In this study, primers that can amplify cnm+/pa+ S. mutans rapidly and specifically using the loop-mediated isothermal amplification (LAMP) method, one of the gene amplification methods, were prepared and conditions were investigated to detect S. mutans in saliva. As a result, the designed primers amplify the cnm and pa genes specifically in a short time. Furthermore, the results of the study using saliva from healthy subjects revealed that approximately one in four individuals carries cnm+/pa+ S. mutans as a NASH hate factor.
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| Free Research Field |
分子生物学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、健常人ボランティアの協力の下、cnm+/pa+ S.mutansの検出および保菌者背景の把握ができるようになり、国内外では初めてとなる研究結果である。また、LAMP法を用いたcnm+/pa+ S.mutansの検出に関する研究も初めてであり、短時間に簡便な検出が可能となれば健康診断等による早期診断が可能となる。すなわち、多くの健常者およびNASH予備軍の人に対して疾病予防として歯科的予防による口腔衛生状態の改善や栄養指導による血管破綻の回避を積極的に行うことができ、将来のQOLの向上に繋がることが期待できる。
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