Development of multi-pulse electroporator using the nanosecond pulse

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K04458
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 21010:Power engineering-related
• Research Institution: Ariake National College of Technology
• Principal Investigator: Kono Susumu 有明工業高等専門学校, 創造工学科, 教授 (30270375)
• Co-Investigator(Kenkyū-buntansha): 冨永 伸明 有明工業高等専門学校, 創造工学科, 教授 (30227631)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: エレクトロポレーション / ナノ秒高電圧パルス / HL-60 / CHO / RAW264.7 / THP-1 / GFP

Outline of Final Research Achievements

A multi-pulse electroporator that combined a commonly used low-voltage millisecond pulses with a high-voltage nanosecond pulse was made as a prototype, and transfection efficiencies of GFP (Green Fluorescent Protein) for both type of cells, adherent cells and floating cells, were investigated.
Experiments were performed under relatively simple pulse conditions and plots based on the cell states were drawn. These plots showed possibilities of transfection for each cell and the pulse conditions resulting in better transfection efficiency.

Free Research Field

パルスパワー工学

Academic Significance and Societal Importance of the Research Achievements

遺伝子などの物質を受精卵や細胞に導入する技術には物理的方法、化学的方法、生物学的方法があるが、導入率・生存率・実験効率（時間効率）・費用・実験者に求められる熟練度などに関して各方法には一長一短がある。これら短所を改善し、技術の向上を目指して研究開発したナノ秒マルチパルスEP装置は，簡単な操作で，さまざまな細胞種に対し効率良い条件設定を容易に見出すことができ，さらに低侵襲な物質導入ができる可能性を持つことが分かった。このような特徴をもつ本装置は，生物学・医学・薬学などの研究に大きく貢献できると考えられる。