2022 Fiscal Year Final Research Report
evelopment of high-efficiency production and purification technology for useful proteins through masking of the interaction interface.
| Project/Area Number |
20K05234
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | サイトカイン / 遺伝子組換タンパク質 / インターロイキン / タンパク質間相互作用 |
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| Outline of Final Research Achievements |
I investigated the effect of co-expression with their receptors on the stability of growth factors with low production yields, aiming to achieve high-efficiency production. Specifically, targeting IL-2, I examined the increase in the expression level of interleukin in silkworms by co-expressing IL-2 and its various receptors using a baculovirus expression system. As a result, I was able to confirm the increase in the expression level of IL-2 in silkworm serum by co-expressing the receptors through Western blotting. However, I did not succeed in developing a separation method between IL-2 and its receptors and were unable to isolate IL-2 as the final product.
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| Free Research Field |
タンパク質工学
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| Academic Significance and Societal Importance of the Research Achievements |
難発現性タンパク質において、結合タンパク質（レセプター）を存在させることで、その安定化を行うことができることを示す成果が得られ、将来的な難発現性タンパク質の高効率生産に繋がる知見が得られたと考える。具体的には、ターゲットとなる難発現性タンパク質とレセプターの界面に変異を導入し、pHや塩条件などマイルドな環境変化に応答してアフィニティを変化できる仕組みを導入することで、ホスト生物内では十分なアフィニティを発揮し、難発現性タンパク質の安定化を行い、精製時に適宜、アフィニティを弱めて、レセプターとの分離を可能とする技術を構築することで、難発現性タンパク質の高効率な発現、精製が可能になると期待される。
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