Development of efficient culture process of paclitaxel with low productivity

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05240
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 27040:Biofunction and bioprocess engineering-related
• Research Institution: Sojo University
• Principal Investigator: YamamotoYamamoto Shinjiro 崇城大学, 生物生命学部, 教授 (40262307)
• Co-Investigator(Kenkyū-buntansha): 宮坂 均 崇城大学, 生物生命学部, 教授 (60451283) ; 林 修平 崇城大学, 生物生命学部, 准教授 (30389522)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 植物細胞培養 / 二相培養系 / パクリタキセル / 疎水性イオン液体 / 疎水性媒体

Outline of Final Research Achievements

Based on our previously obtained data that paclitaxel(PTX) biosynthesis was improved by two-phase culture system using hydrophobic ionic liquids, experiments were conducted to increase the amount of ionic liquid added, and cell growth and PTX production were successfully increased. In addition, PTX productivity could be increased by replacing the ionic liquid with a fresh one in the culture, and the productivity of taxanes containing PTX precursors could also be improved. A two-phase culture system using hydrophobic solids as an alternative medium to ionic liquids was also performed, where polytetrafluoroethylene and polypropylene effectively adsorbed paclitaxel, and polypropylene enhanced PTX productivity. A new culture process utilizing hydrophobic media was proposed.

Free Research Field

生物工学

Academic Significance and Societal Importance of the Research Achievements

本研究は、安全で高効率なパクリタキセルの培養生産プロセスの開発を目指した。疎水性媒体を利用する二相培養系を構築することによって、フィードバック阻害を持つパクリタキセルを疎水性媒体に移動させ、パクリタキセルを効果的に生産させることができた。さらに、疎水性イオン液体を適度な頻度で交換することによってパクリタキセルを飛躍的に培養生産でき、工業的生産の可能性も示唆された。疎水性媒体を利用する二相培養系のバイオプロセスの構築によって高価なパクリタキセルを安価に生産できる可能性も見出されたことから、本研究で得られた研究成果は社会的意義が大きいと考えられる。