2023 Fiscal Year Annual Research Report
Identification of early stage malignancy changes in the structural and biophysical proporties of exosomes by atomic force microscopy (AFM)-based nano-mechanical measurements
| Project/Area Number |
20K05321
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| Research Institution | Kanazawa University |
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Principal Investigator |
YURTSEVER AYHAN 金沢大学, ナノ生命科学研究所, 特任助教 (00761529)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | exosomes / Atomic force microscopy / Nanomechanics / Cells / 3D-AFM |
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| Outline of Annual Research Achievements |
Extracellular vesicles (EVs) are classified into three subclasses, including exosomes, microvesicles (MVs), and apoptotic bodies. Currently, there are no methods to distinguish exosomes from samples containing different EVs. We have developed a method to distinguish exosomes from other extracellular vesicles based on their changed states and mechanical properties, which is crucial for separating exosomes from a heterogeneous sample. We have identified exosomes from heterogeneous EVs using both an anion exchange column based on their surface charge and AFM nanomechanical measurements based on their Young's modulus differences. From the nanomechanical measurements, we found that exosomes have higher Young's moduli than MVs.
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