2023 Fiscal Year Final Research Report
Identification of early stage malignancy changes in the structural and biophysical proporties of exosomes by atomic force microscopy (AFM)-based nano-mechanical measurements
| Project/Area Number |
20K05321
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 29020:Thin film/surface and interfacial physical properties-related
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| Research Institution | Kanazawa University |
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Principal Investigator |
YURTSEVER AYHAN 金沢大学, ナノ生命科学研究所, 特任助教 (00761529)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | Exosomes / 3D-AFM / Nanomechanics |
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| Outline of Final Research Achievements |
We employed 3D-AFM to reveal the structural and associated-nanomechanical properties of exosomes. 3D-AFM showed the presence of distinct nanodomains bulging out from the membrane surface, which can be attributed to the heterogeneous presence of membrane-associated proteins exposed on the vesicles’ outer surfaces. Our results further showed differences between the nanomechanical properties of metastatic and nonmetastatic cancer cell-derived exosomes.
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| Free Research Field |
Scanning probe microscopy (SPM), AFM, Nanoscience
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| Academic Significance and Societal Importance of the Research Achievements |
Cancer is a leading cause of death due to late diagnosis. Distinguishing between metastatic and non-metastatic malignant cell-derived exosomes is crucial. This differentiation aids in understanding exosome functions and enhances their use as biomarkers, improving early cancer detection and outcomes.
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