Development of Medicinal Molecules Releasing Mechanism Driven by Vibrationally Excited Prussian Blue by NIR light

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K05543
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 34010:Inorganic/coordination chemistry-related
• Research Institution: Osaka Metropolitan University (2022-2023); Osaka City University (2020-2021)
• Principal Investigator: Nakajima Hiroshi 大阪公立大学, 大学院理学研究科, 教授 (00283151)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: フェリチン / プルシアンブルー / 過酸化水素還元電極 / バイオセンサー

Outline of Final Research Achievements

In this study, we have successfully developed a method for the ship-in-bottle synthesis of Prussian blue, a cyano-bridged three-dimensional iron complex, in the inner space of the iron ion storage protein ferritin. Prussian blue prepared by a known method readily degraded in higher than neutral pH solutions, restricting its utilization in aqueous solutions. The inclusion of the Prussian blue in ferritin reduced the degradation rate by a factor of 1/100 to 1/1000 and greatly improved the alkaline tolerance of Prussian blue. Based on the improved alkali resistance, we developed a hydrogen peroxide reduction reaction catalyzed by the clathrate of Prussian blue and a method for fixing the clathrate to the gold electrode surface, aiming at fabricating electrode materials for the application of various oxidase enzymes to biosensors.

Free Research Field

生物無機化学

Academic Significance and Societal Importance of the Research Achievements

プルシアンブルーは、着色剤、触媒、セシウムイオンの除去剤など現在も様々な分野で用いられる材料であるが、中性以上のアルカリ性溶液中で容易に分解することが課題であった。本研究は、タンパク質にプルシアンブルーを包摂することでこの問題を解決し、今後のプルシアンブルーの応用領域拡大に貢献できると考える。また、包摂体においてプルシアンブルーがアルカリ耐性を示す機構の解明を通して、プルシアンブルーの分解に関する理解の深化、分解を抑制する分子機構の提案をおこなうことができた。