Development of HPLC columns for highly efficient and selective separation of hydrophilic and ionic substances

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05550
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 34020:Analytical chemistry-related
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: Ikegami Tohru 京都工芸繊維大学, 分子化学系, 准教授 (20301252)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 親水性相互作用クロマトグラフィー / イオン交換 / ミックスモード / 構造選択性 / 分離特性

Outline of Final Research Achievements

Testing methods based on structural selectivity for hydrophilic interaction chromatography (HILIC) and mixed-mode columns were developed. This facilitates the comparison of columns in terms of the hydrophilicity, the degree of hydration, and the weak cation/anion exchange properties of the surface, using a single diagram. Methods were developed to modify silica gel with hydrophilic polymers using different manner of polymerization, and the resulting stationary phases were evaluated by the test method described above. It shows that the retention of hydrophilicity and selectivity can be controlled by adjusting the morphology and modification density of the polymer chains that make up the stationary phase. The columns prepared in this series of studies showed higher selectivity for OH groups than any previous HILIC columns. The conditions for precise separation of sugars were also investigated.

Free Research Field

分離化学

Academic Significance and Societal Importance of the Research Achievements

新しくHILICのカラムを調製して、それがこれまでに報告されたカラムと分離特性（親水性、水和相の厚み、表面のイオン交換性など）と比較し、評価する方法を提供した。このことは、学術的な意義にとどまらず、カラムを製造する企業が製品開発したり、製品の品質管理をしたりする際に利用されうるので、社会的意義が大きいと考える。また、原子移動ラジカル重合（ATRP）や可逆的付加開裂連鎖移動重合（RAFT）を用いて、古典的な重合法では到達できない領域まで、分離媒体の親水性の保持及び構造選択性を向上できることを示した。これは今後の分離媒体開発への指針となることが予想される。