2022 Fiscal Year Final Research Report
White melanosome for the DDS system
Project/Area Number |
20K05703
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 37010:Bio-related chemistry
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Research Institution | Gifu University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
古田 享史 岐阜大学, 工学部, 教授 (40173538)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | メラノソーム / オートファジー / メラニン合成抑制 / 薬物送達 / 構造活性相関 |
Outline of Final Research Achievements |
The compounds that release large amounts of melanosomes were validated, suggesting that they are linked to autophagy. Autophages have been reported to degrade melanosomes and reduce intracellular melanin levels, in this case, a compound reduced melanin levels by promoting melanosome release. In addition, another melanosome release compound was also identified that inhibited the expression and activity of melanin synthase, resulting in low melanin accumulation. The low melanin-containing melanosomes also allowed the identification of fluorescent compounds and their uptake into other cells could be assessed. The melanosomes isolated in this study were taken up not only by keratinocytes but also by macrophages, suggesting their use as seeds for drug delivery.
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Free Research Field |
物質化学
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Academic Significance and Societal Importance of the Research Achievements |
メラノソームが薬物送達の素材として活用できる可能性が示唆された。また、メラノソームをラベルできる化合物は、送達する薬物のリンカーとしても機能することから、今後の改良が期待される。一方で、メラノソームを取り込む細胞が、ケラチノサイトやマクロファージなどの貪食機能が亢進している細胞であったことから、今後は、細胞特異的にメラノソームを導入する手法の構築へと進めていく。また、ケラチノサイトのメラノソーム取り込みは、皮膚の色素沈着にも繋がる。そのため、メラノソームをメラノサイト内の止めておく技術の開発も望まれる。
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