2022 Fiscal Year Final Research Report
Validation of the usefulness of the method for elucidating the interaction between membrane proteins and ligands based on the acquisition of highly efficient revertant strains
| Project/Area Number |
20K05750
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 37030:Chemical biology-related
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 復帰変異株獲得法 / タンパク質リガンド相互作用 / ミトコンドリアの輸送体 / ADP/ATP輸送体 |
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| Outline of Final Research Achievements |
In this study, we tried to develop a new methodology for analyzing the interaction between membrane proteins and ligands, by using a highly efficient revertant acquisition method that we established by ourselves. As a result, we succeeded in getting five mutants of ADP/ATP transporter showing resistance to bongkrekic acid. We tried to further improve the efficiency of this process. As a result, it was possible to create proteins with various single mutations, by subdividing cDNA and introducing mutations into each region with high efficiency.
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| Free Research Field |
生物化学
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| Academic Significance and Societal Importance of the Research Achievements |
タンパク質とリガンドの相互作用様式の解明は薬学や生物化学の研究における大きな課題であり、その効率的な方法論の確立が急務である。研究代表者らは自ら確立した高効率な復帰変異株獲得法を利用することで、リガンドと相互作用しているタンパク質のアミノ酸残基を特定することが可能であることを見出し、本研究ではその有用性の検証と、更なる効率化を目指した実験を遂行した。得られた知見は基礎生物学研究や創薬研究の発展に大きく貢献するもので、その学術的、社会的意義は極めて高い。
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