2022 Fiscal Year Final Research Report
Functional analyses of the malate transporters involved in stomatal closure, and application to crops for environmental adaptation.
Project/Area Number |
20K05772
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 38010:Plant nutrition and soil science-related
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Research Institution | Okayama University |
Principal Investigator |
Sasaki Takayuki 岡山大学, 資源植物科学研究所, 准教授 (60362985)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | 気孔 / ALMT輸送体 / リンゴ酸 / シロイヌナズナ / トマト |
Outline of Final Research Achievements |
Two guard-cell-type ALMT genes, SlALMT11 in tomato and AtALMT12 in Arabidopsis, were compared for stomatal closure patterns between tomato and Arabidopsis plants, as well as for functional domain of the transport proteins. Apoplast malate-induced stomatal closure was observed in both plants, while abscisic acid signaling was different in response to stomatal closure. In contrast, two guard-cell-type ALMT proteins possessed an acidic amino acid at the end of the C-terminus and showed a “bell-shaped” current-voltage pattern as an electrophysiological property. However, truncation of the acidic amino acid from the protein revealed a voltage-dependent increase of the currents. These results suggest that C-terminal acidic residue is involved in the voltage sensing of these proteins.
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Free Research Field |
植物栄養学および土壌学関連
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Academic Significance and Societal Importance of the Research Achievements |
リンゴ酸輸送体ALMTファミリーの生理機能の多様性に着目して,本研究では気孔タイプALMTが関与する,気孔閉口の誘導シグナルにトマトとシロイヌナズナで相同・相違点を見いだした.これは気孔タイプALMTを乾燥耐性の付与,高CO2適応に応用する際に有用な情報となる.さらに,気孔タイプALMTタンパク質のC末端酸性アミノ酸が電位センサーとして機能することは,今後ゲノム編集などでC末端側を削除することで,気孔開口を調節した作物をつくる技術転用への可能性を示した.そして将来的に,気孔タイプALMTの機能制御により,気候変動に耐性を付与した作物をつくり,持続的な作物生産と品質向上への応用が期待できる.
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