Functional analysis of the novel protein that promotes enzymatic degradation of cellulose

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05785
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Shinshu University
• Principal Investigator: Nozaki Kouichi 信州大学, 学術研究院工学系, 准教授 (10313834)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: Cip1 / Trichoderma reesei / LPMO / Cellulose degradation / Cellulase

Outline of Final Research Achievements

It was elucidated the function of a novel protein, Cip1 (Cellulose induced protein 1), which promotes the enzymatic degradation of cellulose. Cip1 promoted the activity of LPMO9A produced by Trichoderma reesei. It was presumed that this facilitates the action of hydrolytic enzymes such as cellulase, promoting cellulose degradation. We clarified that three amino acid residues present in the cleft of the Cip1 protein surface are essential for promoting the reaction, and that the presence of CBM1 increases the activity-promoting effect. The cellulolytic activity of the Cip1 gene disruption strain was reduced by 12% compared to the parent strain. However, by adding Cip1, the degradation activity was recovered and increased to exceed that of the parent strain.

Free Research Field

酵素化学

Academic Significance and Societal Importance of the Research Achievements

本研究により，セルロース分解酵素の活性を促進する新規なタンパク質Cip1の機能を明らかにすることができた。Cip1がLPMO9Aの分解能力が促進することで，セルラーゼなどの加水分解酵素の反応が相乗的に促進されていることを明らかにした。市販セルラーゼ製剤においてもCip1を添加することで，その分解力を向上できる可能性が期待された。