2022 Fiscal Year Annual Research Report
Metabolomics-driven approach to understand antibiotics induction ability mechanism in Streptomyces coelicolor
| Project/Area Number |
20K05787
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| Research Institution | Osaka University |
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Principal Investigator |
PUTRI SASTIA 大阪大学, 大学院工学研究科, 准教授 (50729796)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | Streptomyces coelicolor / antibiotics production / metabolomics / antibiotics control |
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| Outline of Annual Research Achievements |
Gamma-butyrolactones (GBL) plays a big role on S. coelicolor antibiotic production. Among the GBL, SCB1 acts in a highly specific manner to elicit the production of actinorhodin (ACT, blue color) and undecylprodigiosin (RED, red color). The SCB signaling system includes the signaling molecule (SCB1), signaling molecule synthase protein (ScbA), and receptor/gene regulator (ScbR). A previous study tried to analyze SCB1 and the SCB signaling system through knockout of the involved genes including the scbA gene (strain M751) and the scbR gene (strain M752). Streptomyces coelicolor of wild-type strain M145 and two mutants M751 and M752 were cultivated to examine their growth, antibiotics production (CPK, RED and Act), and metabolite profiles. Three growth stages of the Streptomyces strains were distinguished, including the lag phase, exponential growth phase, and stationary phase. Based on metabolome data, strain M751 showed distinct profile at 44 h cultivation time (early stationary phase), which may represent different precursors needed to produce certain antibiotics. Strain M751 markedly overproduced actinorhodin 3-folds higher than the M145 (wild-type) and the production continued to increase until 80 hours. Metabolites that showed accumulation in M751 included allantoic acid, glycine, isoleucine, leucine, and trehalose. The GC-MS based metabolomic study on S. coelicolor documented trend of metabolite changes upon GBL mutation and antibiotic production. Correlation between observed metabolites unique to M751 to antibiotics control will be a subject of future studies.
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