2022 Fiscal Year Final Research Report
Exploration of the novel function of the ribosome in tolerance to chronic DNA damage stress
| Project/Area Number |
20K05790
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Gakushuin University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | リボソーム |
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| Outline of Final Research Achievements |
Investigation of the effects of DNA damage stress on the ribosomes of budding yeast showed that DNA damage stress reduces the translational activity of ribosomes and that under DNA stress conditions there may be ribosomal proteins that are replaced by paralogous proteins. In addition, deletion mutant of ribosomal protein gene recovered their growth rate by aneuploidy of the chromosome encoding the paralog protein, thereby reducing their DNA damage stress tolerance. In addition, it was found that ribosomal protein deficiency increases the susceptibility to chromosomal partitioning abnormalities, resulting in an increased frequency of suppressor mutant strains.
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| Free Research Field |
分子遺伝学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、DNA損傷ストレスがリボソームの活性や複合体の構成に影響を与えることが明らかになった。この成果はこれまであまり注目されていなかったリボソームとゲノム安定性との関係を示すものであり、新たな研究分野に発展する可能性を秘めている。また、リボソームタンパク質の欠損が染色体分配異常を引き起こすとともに、パラログ遺伝子の重複により細胞内翻訳活性を補完するという新たな発見は、染色体恒常性維持にリボソームが重要な役割を果たすことを示すとともに、異数化による遺伝子機能相補の一般性を示唆するものである。
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