2022 Fiscal Year Final Research Report
Epistatic studies of bacterial adaptive growth by two component system
| Project/Area Number |
20K05795
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Hosei University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
吉村 美歩 法政大学, 生命科学部, 助手 (40865820)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 細菌 / 適応増殖 / 環境応答 / 大腸菌 / 二成分制御系 / ゲノム編集技術 / ゲノム微生物学 / 代謝-遺伝子発現制御ネットワーク |
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| Outline of Final Research Achievements |
The aim of this study was to clarify the contribution of stress response to individual survive and adaptive growth of Escherichia coli. Two-component regulatory system (TCS) is a conserved signal transduction system in prokaryote. TCS consists of a pair of a histidine sensor kinase (HK) and a response regulator (RR). The genome sequence of E. coli K-12 predicts 30 HK and 34 RR genes. Genome editing HoSeI method isolated the all 34 RR genes knockout and the all 30 SK gene knockout E. coli strains. The genome sequencing, transcriptome, proteome, metabolome, and growth were investigated in these strains, indicating that RRs were required for survival under several stress conditions, whereas HKs were unnecessary for both survival or adaptive growth. The metabolite profiles of all SK gene knockout strain differed significantly from those of the parent. These results show that several regulators are activated with phosphate metabolites in the HK-independent manner for individual survival.
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| Free Research Field |
応用微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
環境応答や適応増殖にTCSの「センサーが不要」で「レギュレーターは低分子のリン酸化有機化合物で活性」し、「センサー遺伝子をもたない細胞に特異的な代謝産物プロファイル」があることを見出した。これらの成果は、細菌生存に、代謝と連携し活性化するレギュレーターが本質的に重要であることを示し、進化的にどのようにセンサーを獲得したのかという新しい生物学的問いを与え、自然環境で増殖せず生存する細菌の理解に貢献する。TCSは、病原性細菌の病原性、抗菌剤耐性、バイオフィルム形成に関わる遺伝子群を発現する。生存と増殖ではたらく代謝-TCSレギュレーターは、抗菌薬開発や多剤細菌感染症における新しい標的になる。
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