2023 Fiscal Year Final Research Report
Approaches to transcription factor research in industrial microorganisms at molecular level.
| Project/Area Number |
20K05802
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | タンパク質工学 / 転写因子 / タンパク質発現・精製 |
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| Outline of Final Research Achievements |
The molecular mechanisms that regulate the expression of microbial enzymes are often a black box, and this is one of the factors that hinder the application of microorganisms. In this study, we aimed at functional and structural analysis of FlbC, a transcription factor of Aspergillus oryzae, and first constructed an expression system using E. coli. We succeeded in obtaining 12 milligrams of protein per liter of E. coli culture. Crystallization was attempted, and crystals were obtained, but the quality of the crystals was found to be insufficient for analysis. On the other hand, since purified FlbC was obtained, in vitro analysis could proceed. Gel-shift electrophoresis analysis confirmed that FlbC has the ability to bind to specific DNA sequences.
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| Free Research Field |
構造生物学
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| Academic Significance and Societal Importance of the Research Achievements |
微生物のタンパク質発現を司る転写因子について、特に麹菌においては、これまでにタンパク質発現系、精製系の構築に成功した例がなく、本研究で初めて機能を有した形で大量の転写因子タンパク質の獲得に成功した。学術的には、タンパク質の大量発現系構築に成功したことは、今後の転写因子研究に大きく寄与する。また、産業的な成果として期待されることは、タンパク質発現システムを、科学的なエビデンスに基づいてデザインすることで、より効率的なタンパク質生産システムを構築するための基盤研究へと発展することである。
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