Development of enzymatic conversion using Rhodococcus cells expressing heterologous genes

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05805
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Gifu University
• Principal Investigator: YOSHIDA Toyokazu 岐阜大学, 工学部, 教授 (90220657)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 酵素変換 / Rhodococcus / 異種遺伝子発現

Outline of Final Research Achievements

Rhodococcus host was suitable for expression of heterologous genes with high GC content, and the enzyme maintained stable activity in the host cells. Rhodococcus cells were resistant to organic solvents, and the enzyme expressed in the cells maintained higher activity in reactions in the presence of organic solvents than in the case of the E. coli host. In the conversion of substances by the Rhodococcus host, the enzyme activity remained stable even in a long reaction time, and substrate transformation at high concentrations, which could not be obtained with the E. coli host, was possible.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

ロドコッカス宿主は高GC含量の異種遺伝子の発現に優位であるだけでなく、その細胞は堅牢かつ有機溶媒耐性を示した。従来は大腸菌細胞が汎用されてきたが、ロドコッカス宿主は酵素による物質変換における新たなツールとして位置づけられる。NADPHの再生系を内在していることから、酵素による物質生産という応用面でも利用価値が高いことが判明した。