Analysis of NO signalling and NO homeostasis in actinomycetes.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05811
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: Sasaki Yasuyuki 東京農業大学, 生命科学部, 准教授 (50398814)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: actinobacteria / Streptomyces / nitric oxide / signalling / homeostatic regulation

Outline of Final Research Achievements

The present study suggests a partial molecular mechanism for nitric oxide signalling and homeostasis in Strepotomyces coelicolorA3(2). Molecular mechanisms of NO-induced antibiotic production and morphological differentiation in this bacterium were suggested. Furthermore, mechanisms of NO homeostasis were suggested, including control of the concentration of endogenous NO by the low-molecular-weight thiol compound mycothiol and stabilization of the activity of NO-metabolising iron-containing protein groups by NO-dependent iron uptake.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

本研究では、Strepotomyces coelicolorA3(2)の一酸化窒素シグナリング、及び恒常性維持機構の一部の分子レベルでのメカニズムが示唆された。NOによる抗生物質、形態分化の制御についての報告はこれまでになく、Streptomyces属細菌を利用した工業的生産プロセスおよび生産性を、生育制御の観点から改善する新しい試みの展開が期待される。