2024 Fiscal Year Final Research Report
Creation of novel amino acid metabolizing enzymes by genome information and protein engineering
| Project/Area Number |
20K05816
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Osaka Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2025-03-31
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| Keywords | ゲノム情報 / 酵素 / 酵素の機能と構造 / アミノ酸代謝酵素 / 酵素の新規機能創製 / 酵素応用 |
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| Outline of Final Research Achievements |
The main objective of this study was to find out novel enzyme genes functioning in amino acid metabolisms based on genomic information, and to promote the industrial application of those enzymes after the production and the structural and functional analyses, improvement of functions. As the results, L-arginine dehydrogenase was discovered, and the function and structure of the enzyme were analyzed.it was successfully applied to a novel enzyme analysis for L-arginine. In addition,two distinct alanine dehydrogenase genes were identified in thermophilic spore-forming bacterium, the one functioning in nutrient-growing cells and the other in spores. Furthermore, several new enzymes, including a novel thermostable leucine dehydrogenase and a meso-diaminopyrimidine dehydrogenase, were identified, and their functions and structures were analyzed.
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| Free Research Field |
応用生物化学
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| Academic Significance and Societal Importance of the Research Achievements |
近年、微生物の膨大なゲノム情報から新規な目的酵素遺伝子を見出すことが容易になっている。本研究では、ゲノム情報をもとに、産業応用面で有用性期待できるアミノ酸代謝系の種々の酵素遺伝子を見出し、遺伝子の大腸菌での発現と生産、精製、機能と構造解析とそれらに基く機能改変を行い、新しい機能をもつ、酵素の創製とその応用面の開発を進めることを主な目的とする。その成果は、食品分析、医療分析、環境分析、医薬品の製造など、新しい応用開発への展開が期待でき、学術的、社会的意義は大きい。
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