2023 Fiscal Year Final Research Report
Analysis of the response mechanism for fruiting body induction in basidiomycetes and its effective application to mushroom production
| Project/Area Number |
20K05828
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38030:Applied biochemistry-related
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | 担子菌 / 子実体形成 / 遺伝子発現 / ラッカーゼ / 酵素機能 |
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| Outline of Final Research Achievements |
In this study, we constructed a heterologous expression system for the laccase isozyme in response to low temperature, which is significant in the fruiting body formation of Flammulina velutipes (enokitake), and analyzed various properties of the prepared enzyme. In addition, overexpression of this isozyme in F.velutipes suggested that the enzyme contributes to the growth enhancement of mycelial growth and fruiting body formation. Furthermore, information on orthologs that respond to fruiting body formation condition was acquired.
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| Free Research Field |
微生物生化学
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| Academic Significance and Societal Importance of the Research Achievements |
担子菌において子実体（キノコ）は、外的刺激に応答して形成され、食用担子菌においては可食部となるだけでなく、生理活性物質を含有する種もあることから有用物質の供給源ともなり得る産業上重要な器官である。よって、本研究で着目したラッカーゼアイソザイムをファクターとするエノキタケの成長促進効果や子実体形成条件に応答する遺伝子オルソログに関する情報は、さらなる解析は必要であるものの、学術的意義のみならず社会的にも重要な意義を持つものと考えられる。
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