2022 Fiscal Year Final Research Report
Preparation of microbial broth library and development of multivariate screening system toward new active compounds search
| Project/Area Number |
20K05858
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38040:Bioorganic chemistry-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
NOGAWA Toshihiko 国立研究開発法人理化学研究所, 環境資源科学研究センター, 技師 (40462717)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 微生物二次代謝産物 / ブロスライブラリー / 新規活性物質 / 多変量スクリーニング |
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| Outline of Final Research Achievements |
In this program, I had focused on search and isolation of new secondary metabolites from microbes. To achieve the goal, the following 5 tasks were carried out: (1) preparation of a microbial broth library using different culture conditions, (2) LC/MS and TLC analyses of the library to obtain physicochemical properties, (3) evaluation of biological activities of the library, (4) multivariant analysis based on physicochemical properties and activity profile, (5) isolation and identification of active metabolites. About 1,500 broths were prepared from actinomycetes and fungi to construct the library. It was subjected to LC/MS and TLC analyses and biological activity tests. Based on the results, characteristic broths were selected. They afforded structurally new metabolites with biological activity. The results were published and presented in academic fields. These results showed advantages of this approach to search and isolate structurally new metabolites with biological activity.
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| Free Research Field |
天然物有機化学
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| Academic Significance and Societal Importance of the Research Achievements |
微生物二次代謝産物は、多様な構造や生物活性から重要な化合物群である。しかし、その探索研究には精製の手間や時間、最近では新規化合物発見の難しさなどの問題がある。そのため、有用性は理解されながらもコストや成果の得にくさなどの面から研究の継続が困難になっている。本課題では微生物の二次代謝産物生産能の有効利用に着目し、複数の探索方法の組み合わせにより新規化合物群の探索を行った。本課題を通して確立した方法により新規活性物質を有効に探索することができ、医薬品などのシード探索やバイオプローブの創出につながることが期待できる。さらに、限りある微生物資源の有効活用においても重要である。
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