2022 Fiscal Year Final Research Report
Distribution of novel nitro group-forming enzymes in plants and structures involved in substrate recognition
| Project/Area Number |
20K05861
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38040:Bioorganic chemistry-related
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| Research Institution | Toyama Prefectural University (2021-2022)
University of Tsukuba (2020) |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | ビワ / ニトロ基合成酵素 / シトクロムP450 / 脂肪酸水酸化酵素 / CYP94A / 植物特化代謝物 |
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| Outline of Final Research Achievements |
Plants biosynthesize numerous numbers of plant specialized metabolites, but few contain nitro groups. We have discovered a novel nitro group forming enzyme CYP94A90 from loquat. CYP94A family members are widely distributed in dicot plants and some of them have been characterized as fatty acid hydroxylases. In this study we evaluated fatty acid hydroxylase activity and nitro group-forming activity of CYP94A from loquat and other plants. Our results demonstrated that CYP94As are “promiscuous” fatty acid hydroxylase, catalyzing the formation of nitro groups from aldoximes, and are widely distributed in dicot plants. In addition, CYP94A exhibited a higher affinity for fatty acids than aldoxime. Thus, CYP94A was thought to catalyze nitro group synthesis reactions under special conditions in which aldoxime accumulates at high concentrations.
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| Free Research Field |
生化学
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| Academic Significance and Societal Importance of the Research Achievements |
希少な天然物の生合成酵素は、進化の過程で特定の反応を触媒するように最適化され、特定の種だけが有している場合が多い。しかし、本研究では植物に広く存在している酵素が特定の条件で本来の機能とは異なる酵素活性を発揮し、極めて珍しい化合物の生合成に関わることを示唆した。植物特化代謝物の多様化機構の新たな側面を提示した点で学術的意義がある。
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