2022 Fiscal Year Final Research Report
Analysis of apoptosis induction on the inactivation by low pressure carbon dioxide microbubbles
| Project/Area Number |
20K05896
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38050:Food sciences-related
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| Research Institution | Nippon Veterinary and Life Science University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 殺菌 / 加圧二酸化炭素 / マイクロバブル |
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| Outline of Final Research Achievements |
Increase of cell surface hydrophobicity and decrease of intracellular pH and mitochondrial membrane potential in Saccharomyces pastorianus were induced by low-pressure carbon dioxide microbubbles (CO2MB) at 35℃. Cell membrane fluidity in S. pastorianus was changed by CO2MB over 40℃, resulting in the leakage of nucleic acid and protein. Soluble proteins in S. pastorianus cells were insolubilized by CO2MB and one of those proteins was glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme. It was suggested that active oxygen in S. pastorianus cells was generated by CO2MB because of the increase of AP site. The number of surviving S. pastorianus cells treated by CO2MB at 35℃ and 40℃ increased gradually during storage at 25℃ but at 45℃ and 50℃ remained undetected during storage of 3 weeks.
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| Free Research Field |
食品科学
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| Academic Significance and Societal Importance of the Research Achievements |
低加圧二酸化炭素マイクロバブル（CO2MB）は、従来の加熱よりも低い温度帯または短時間で殺菌可能であるため、近年多様化した食品への適用も期待できる見込みのある殺菌技術であるが、その殺菌メカニズムがわかっていない。今回の研究において、CO2MBにより微生物細胞内で生じる様々な変化を明らかにし、特に変性したタンパク質の1つが解糖系酵素であったことを特定することができた。この結果は、世界中で行われている加圧CO2殺菌の研究において非常に重要な成果であり、他の殺菌技術の殺菌メカニズム解析にも役立つものである。
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