Mycobacterial infection to farm-raised fishes and evaluation of food safety

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K05899
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38050:Food sciences-related
• Research Institution: Tezukayama University
• Principal Investigator: Fujiwara Nagatoshi 帝塚山大学, 現代生活学部, 教授 (80326256)
• Co-Investigator(Kenkyū-buntansha): 前田 伸司 北海道科学大学, 薬学部, 教授 (50250212) ; 綾田 稔 大阪公立大学, 大学院医学研究科, 研究員 (90222702)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 抗酸菌 / Mycobacterium / 養殖魚 / 脂質生化学

Outline of Final Research Achievements

M. pseudoshottsii, M. shottsii and M. marinum are closely related M. ulcerans, and are the causative bacteria of the fish infections. We separated and collected many clinical isolates from infected fishes and identified the bacterial species. The lipid fraction was extracted from these clinical isolates and evaluate the heterogeneity of lipids. The structures of mycolic acid subclasses and molecular species were confirmed, and the function of the M. marinum madR-KO mutant. Cord factor (trehalose-6,6'-dimycolate) expresses in common, and phenolglycolipid and mycolactone were heterogeneity. It was suggested that these glycolipids affect the host immune responses.
The epidemiological and lipid-biochemical studies of the acid-fast bacteria infected in aquaculture fish were contributed to food safety.

Free Research Field

細菌学、脂質生化学

Academic Significance and Societal Importance of the Research Achievements

養殖魚における抗酸菌感染症の全貌を疫学的観点から理解し、抗酸菌感染魚の脂質免疫学的特徴を明らかにすることで、病原性、感染性との関連を検討した。本研究から、総合的な魚類抗酸菌感染症の現状と予防対策を検討し、食の安全性に資する基礎的知見が得られた。