2023 Fiscal Year Final Research Report
Study on exploration of new crop-specific and multi-copied DNA markers using bioinformatic approach
| Project/Area Number |
20K05901
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38050:Food sciences-related
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
Soga KEISUKE 国立医薬品食品衛生研究所, 生化学部, 主任研究官 (50746336)
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| Co-Investigator(Kenkyū-buntansha) |
吉田 光範 国立感染症研究所, ハンセン病研究センター 感染制御部, 主任研究官 (70772630)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | バイオインフォマティクス / 遺伝子検査 / リアルタイムPCR / コメ / とうもろこし / マルチコピー / DNAマーカー / 食品分析 |
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| Outline of Final Research Achievements |
Genetic testing methods such as PCR targeting species-specific and multi-copy DNA markers are useful for detecting adulteration, contamination in processed foods and crops in vomit.
In this study, we investigated the maize-specific and multi copied markers targeting ribosomal RNA. We also investigated the rice-specific and multi copied markers using bioinformatics approaches, and a search pipeline was developed. Highly sensitive real-time PCRs were successfully developed by targeting the respective candidate marker regions. On the other hand, non-specific and trace amplification could not be completely eliminated in certain crops, and further studies are required when applying them to tests.
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| Free Research Field |
食品科学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で開発された高感度リアルタイムPCR法を用いることで、加工食品中のとうもろこしおよびイネの混入の確認が可能と考えられる。この場合は、食品偽装食品中のアレルゲン性の確認等の検査への要用が期待できる。また、高感度かつ特異的マーカー探索パイプラインは、今回検討した作物以外の作物への応用も期待でき、食品遺伝子検査の高感度化に貢献できると考えられる。
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