Development of technology for isolation and cultivation of probiotic bacteria using metabolites from in vitro colonic microbiota culture

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05938
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38050:Food sciences-related
• Research Institution: Kobe University
• Principal Investigator: Sasaki Daisuke 神戸大学, 科学技術イノベーション研究科, 特命助教 (00650615)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 腸内細菌 / マイクロバイオーム / 酪酸産生菌 / 短鎖脂肪酸 / 酪酸 / in vitro腸内細菌培養 / 分離培養

Outline of Final Research Achievements

Construction of a useful colonic bacteria isolation method was completed.
First, healthy human feces containing many useful bacteria candidates were cultured in vitro. Subsequently, a new medium was developed by adding the culture supernatant after cultivation to the anaerobic synthetic medium. As a result, more than 300 isolated strains were isolated and cultured. Bacterial species were identified by sequencing the 16S rRNA gene, and many of the bacteria belonged to the phyla Bacteroidetes and Firmicutes, and a small number of bacteria belonged to the phyla Actinobacteria and Proteobacteria.
Among them, several species of butyrate-producing bacteria, including uncultured strains, which are thought to be beneficial to human health, have been isolated and cultured.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

ヒトの腸内細菌の変化がさまざまな疾患の原因や結果になっていることが明らかにされてきている中、健康維持、恒常性維持、免疫調整に深く換喩することが判明している酪酸を産生する腸内細菌を実際の腸内から分離し、それを利用した生菌製剤の研究開発が求められてきている。本研究はそのような将来的に注目される研究開発のために、その材料を提供する培養方法として、今後非常に重要になる可能性がある。