2022 Fiscal Year Final Research Report
Generation of phosphate starvation tolerant rice using genome editing and analysis of gene function
| Project/Area Number |
20K05973
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 39010:Science in plant genetics and breeding-related
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | イネ / リン酸代謝 / ゲノム編集 |
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| Outline of Final Research Achievements |
Phosphorus is essential for plant body building and energy production, but we rely on imports for the supply of phosphate fertilizers, and there are concerns about supply shortages due to world situations.In order to realize low-phosphate-input agriculture, we have to elucidate the response mechanism of plants to phosphate starvation. We generated functional mutants of phosphate homeostasis regulators (OsSPX1, OsSPX3, and OsNLA1) by CRISPR-Cas-based genome editing technology in rice(Oryza sativa). The resulting functional mutant grew well even under low-phosphate cultivation conditions, and the amount of phosphate in the tissue was basically the same as that of the wild type.
Since these are mutants generated by genome editing technology, they can be used as genetic resources for low-phosphate breeding.
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| Free Research Field |
植物のゲノム生物学、ゲノム塩基配列解読、植物のゲノム編集
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| Academic Significance and Societal Importance of the Research Achievements |
SPXドメイン（植物のリン酸恒常性を保持する蛋白質等に共通に存在）を有するタンパクの機能解析は組換え体やトランスポゾン転移による突然変異体が主であり、ゲノム編集を用いるアプローチは、これまで行なわれていなかった。今回作出した遺伝子の機能変異体は点突然変異等の限定領域変異であり、遺伝子のみの変異効果が機能に与える影響を調査することができた。
また得られた機能変異体は低リン酸栽培条件下でも生育し、組織内リン酸の量も野生型と変わらなかった。これらがゲノム編集体であることを考慮すると、将来低リン酸育種のための遺伝資源として利用されうる。
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