Analysis of the relationship of the self-incompatibility stigma factor, SRK, between the intensity of the self-incompatibility in Brassicaceae

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05979
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: Tohoku University
• Principal Investigator: Yamamoto Masaya 東北大学, 農学研究科, 准教授 (70732543)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 自家不和合性 / アブラナ科 / 受容体キナーゼ / SRK

Outline of Final Research Achievements

Self-incompatibility of Brassica vegetables is an important agronomic trait related to breeding and seed production. It has been known that the intensity of self-incompatibility varies between strains and varies depending on the cultivation environment, but the molecular mechanism has remained unclear. In this study, we examined the intensity of self-incompatibility of Brassicaceae plants by focusing on SRK, the stigmatic factor of self-incompatibility. As a result of this study, we found that the intensity of self-incompatibility is stable in the S strains and that it correlates to some extent with the plasma membrane localization of SRK. In addition, we were able to confer the self-incompatibility recognition specificity of B. rapa to Arabidopsis thaliana, as an experimental model plant.

Free Research Field

植物分子生物学

Academic Significance and Societal Importance of the Research Achievements

流通するアブラナ科野菜のほとんどは異なる親株の後代であるF1品種であり、その採種の一部には自家不和合性が利用されている。しかし、自家不和合性程度の低さによる自殖種子混入が問題となっている。本研究では、自家不和合性程度が安定的なS遺伝子系統を見いだした。見いだした安定的な系統を利用することで自家不和合性程度が安定で高F1純度を達成できる品種の育成に寄与する。
また、シロイヌナズナでB. rapaの認識特異性を付与する実験系を構築できたため、今後、B. rapaで蓄積した知見とシロイヌナズナの実験の簡易さを利用することで、自家不和合性研究の加速が期待できる。