Visualization of cell cycle in phytopathogenic fungus Pyricularia oryzae

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06052
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Kobe University
• Principal Investigator: Ikeda Kenichi 神戸大学, 農学研究科, 准教授 (40437504)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: いもち病菌 / Pyricularia oryzae / Magnaporthe oryzae / 付着器 / 細胞周期 / オートファジー

Outline of Final Research Achievements

In order to evaluate the cell cycle of rice blast fungus, I modified the commercially available Fucci system for rice blast fungus and succeeded in obtaining mutant strains. When the cell cycle was evaluated using these mutant strains, it was found that the cell cycle was one cycle in the process of appressorium formation in the blast fungus. In contrast, no change was observed on the hydrophilic surface, and the cell cycle was accelerated on the plasma-irradiated petri dishes similar to root infection. These cell cycle changes were consistent with the evaluation of the nuclear division pattern using an RFP-labelled histone H1 strain.

Free Research Field

植物保護

Academic Significance and Societal Importance of the Research Achievements

いもち病菌は植物病原糸状菌においてモデルとなっており、その複雑な病原性発現機構を理解することは、病害防除に向けて重要である。胞子が発芽する過程において、どのように細胞周期を調節しているのかを明らかとすることにより、外部環境をどのように感知し、どのような仕組みで制御されているのかを明らかとするために、まずは細胞周期をモニタリングできる技術の構築を試みる。