Studies on the mechanism of the activation of innate immunity of kuruma shrimp by photosynthetic bacteria

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06216
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 40030:Aquatic bioproduction science-related
• Research Institution: Sojo University
• Principal Investigator: Miyasaka Hitoshi 崇城大学, 生物生命学部, 教授 (60451283)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: photosynthetic bacteria / kuruma shrimp / probiotics / innate immunity

Outline of Final Research Achievements

Rhodovulum sulfidophilum KKMI01, a marine PNSB is a marine PNSB isolated from the seawater collected in Kami-Amakusa, Kumamoto, Japan. It has a superior effect as probiotics for shrimp. R. sulfidophilum KKMI01 was the first strain of PNSB demonstrated, at the level of gene expression, that it stimulates the innate immunity of shrimp. Significant upregulations of the genes of proPO, SOD2, HPS70, Dph7 (diphthamide biosynthesis 7), and Serpin, a serine protease inhibitors, were observed in kuruma shrimp (M. japonicus) by the treatment of R. sulfidophilum KKMI01. This strain was the first probiotic microorganism proven to promote shrimp growth at the level of gene expression, and the effective dosage of this strain was much lower compared to other probiotic microorganisms.

Free Research Field

生物工学

Academic Significance and Societal Importance of the Research Achievements

（学術的意義）微生物（プロバイオティクス菌）によってエビの成長が促進されるという報告は過去40年間で約60報ある。本研究では海産性光合成細菌Rhodovulum sulfidophilum KKMI01投与によりエビの成長に直接関与するクチクラ合成、筋肉合成に関わる遺伝子発現が高まることを示した。これは、プロバイオティクス菌の効果を遺伝子発現レベルで証明した世界初の例である。
（社会的意義）海産性光合成細菌Rhodovulum sulfidophilum KKMI01はすでにクルマエビ洋食の現場で利用されている。養殖業者には収益増、消費者には良質なクルマエビの提供で貢献している。