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2022 Fiscal Year Final Research Report

Analyses of shellfish hematopoiesis using single-cell RNA-Seq and genetic engineering technology

Research Project

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Project/Area Number 20K06222
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionTohoku University

Principal Investigator

Nagasawa Kazue  東北大学, 農学研究科, 助教 (50794236)

Co-Investigator(Kenkyū-buntansha) 横井 勇人  東北大学, 農学研究科, 助教 (40569729)
尾定 誠  東北大学, 農学研究科, 教授 (30177208)
Project Period (FY) 2020-04-01 – 2023-03-31
Keywords貝類 / 造血機構 / 幹細胞 / シングルセル解析 / 遺伝子改変 / プロモーター / 強制発現系
Outline of Final Research Achievements

To elucidate the mechanisms of hematopoiesis and hemocyte differentiation in bivalves, cytological and bulk RNA-Seq analyses of scallop hemocytes were performed to clearly identify hemocytes cells as immune cells by using novel bivalve hemocyte markers. In addition, scallop hemocytes were classified into 10 different cell populations by single-cell RNA-Seq approach. Next, to develop gene engineering technology in bivalves, we constructed a microinjection system optimized for multiple species of marine bivalves and successfully expressed GFP in fertilized eggs. Furthermore, we discovered a novel viral promoter that is highly expressed in scallop primary culture cells, and succeeded in gene delivery to primary culture cells using electroporation.

Free Research Field

貝類生理学

Academic Significance and Societal Importance of the Research Achievements

本研究では、日本の重要な養殖対象種であるホタテガイの生体防御の要である血球について、細胞学的特徴や免疫細胞としての性質を初めて明らかにし、水産増養殖業の防疫対策に資する成果を得た。さらにこれまで、遺伝組換え等の技術が全く開発されてこなかった二枚貝に対し、本研究では外来遺伝子を強制的に発現させることが可能な新規ウイルスプロモーターを発見した。以上、本研究の成果と開発された技術は、二枚貝や他の軟体動物の遺伝子工学を大きく発展させるものであり、学術的に重要な成果である。

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Published: 2024-01-30  

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