Behavior of antimicrobial peptides in kuruma shrimp during antiviral response

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06247
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 40040:Aquatic life science-related
• Research Institution: Okayama University of Science (2022); Fisheries Research and Education Agency (2020-2021)
• Principal Investigator: Mekata Tohru 岡山理科大学, 獣医学部, 准教授 (40597944)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 抗微生物性ペプチド / クルマエビ / ウイルス感染

Outline of Final Research Achievements

In order to understand the behavior of antimicrobial peptides among the factors related to the antiviral response in the hemolymph of shrimp, the identification of these factors and the construction of a detection system were conducted. After collecting the hemolymph from shrimp that had induced an antiviral response and removing Highly Abundant Proteins by ultrafiltration column, the detection of anti-microbial peptides was attempted by mass spectrometry and genetic analysis. The analysis revealed that C-type lysozyme and several anti-LPS factors were detected in the hemolymph. Furthermore, the expression levels of all factors were increased during antiviral response, suggesting that these factors play an important role during viral infection.

Free Research Field

魚介類免疫学

Academic Significance and Societal Importance of the Research Achievements

エビ類の養殖では様々なウイルス病が蔓延しており、一刻も早い防除対策が世界的に望まれている。このような疾病の被害を未然に防ぐために、免疫賦活剤や感染防除対策に関する研究開発が行われている。このような研究の中で、エビ類の抗ウイルス免疫能を向上させる物質が発見されてはいるが、その作用機序は不明なままである。このような免疫賦活剤の実用化を進めていく上でも、その作用機序の解明が望まれている。