Cultivation and diversity of syntrophic acetate-oxidizing bacteria

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06336
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 41050:Environmental agriculture-related
• Research Institution: Yamagata University
• Principal Investigator: Hattori Satoshi 山形大学, 農学部, 准教授 (40373352)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 酢酸酸化細菌 / 還元的酢酸生成細菌 / メタン生成古細菌 / 微生物菌叢解析 / 分離培養

Outline of Final Research Achievements

In this study, microbial diversity in peat pond was studied by using a culture-independent 16S rRNA gene amplicon analysis. The results showed that several bacterial families (Clostridiaceae, Ruminococcaceae, Veillonellaceae, Holophagaceae, and Spirochaetaceae) to which previously reported homoacetogens belonged were detected. In addition, cultivation of the syntrophic acetate-oxidizing bacteria was attempted. Although the attempt was not successful, several homoacetogens and other bacteria have been successfully cultivated and isolated. The 16S rRNA gene analysis showed that one strain related to the homoacetogens was likely to be phylogenetically novel at the species-level. Also, the other strain was considered as belonging to a novel family of the order Bacteroidales. The findings in this study will contribute to the studies of microbial diversity and function in the peat ecosystems.

Free Research Field

微生物学

Academic Significance and Societal Importance of the Research Achievements

酢酸はメタン生成環境下において複数の微生物群による分解過程で生成する化合物であり、これらの蓄積は嫌気的有機物分解プロセスの破綻を招くことから、その分解あるいは生成を担う微生物は非常に重要である。本研究では酢酸の生成に関与する細菌として、新規の還元的酢酸生成細菌の取得に成功した。これらの成果は泥炭環境下におけるメタン生成プロセスにおいて知見の乏しかった当該細菌の多様性や機能解明に貢献するものと思われる。