2023 Fiscal Year Final Research Report
Elucidation of calcium signaling mechanism in thermotaxis of bovine sperm and its application to improvement of conception rate
| Project/Area Number |
20K06362
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 42010:Animal production science-related
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高木 優二 信州大学, 学術研究院農学系, 准教授 (20226757)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | ウシ精子 / 走温性 / 温度勾配 / 制御因子 / 発現機構 / 運動解析 |
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| Outline of Final Research Achievements |
This study is basic research for applying a sperm evaluation method using thermotaxis to livestock production sites. Thermotactic sperm in the low temperature area of the temperature gradient decreased their curvilinear velocity and increased shear angle as they moved to the high temperature area. We confirmed thermotaxis in goat sperm. Sperm calcium ion levels in the temperature gradient area and constant temperature area were different; sperm in the temperature gradient area increased their calcium concentration after 5 seconds and maintained it until 30 seconds later. We confirmed the involvement of TRPV3, 4 channels, and potassium channels in changes in the motility of thermotactic sperm. We confirmed the expression of thermotaxis in spermatozoa in a viscous medium, and found that sperm expressing thermotaxis at high temperatures tended to have a high capacitation rate. It was suggested that hyperactivated motile sperm may adapt to the viscous medium and change their motility.
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| Free Research Field |
家畜繁殖
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は精子の走温性における運動調節とカルシウム作用機構を調べた。走温性発現精子の運動性の変化、ヤギ精子の走温性が明らかになり、走温性発現、運動調節機構の一部が解明され、カルシウム作用機構の解析から、温度勾配における精子のカルシウムイオンレベル、走温性発現精子の運動性におけるTRPV、カリウムチャネルの関与が明らかになり、走温性発現経路、伝達機構の一部が解明される。走温性発現精子の受精能の解析から、粘性培地における走温性の発現、CTC染色精子の受精能が解明される。走温性を指標にした精子の受精能の解析、雄ウシの繁殖能力の評価が可能になり、効率的なウシの増産が可能になり、畜産分野に貢献できる。
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