Regulation factors of differentiation and growth in bovine in vitro fertilization embryos.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06373
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42010:Animal production science-related
• Research Institution: Iwate University
• Principal Investigator: Sawai Ken 岩手大学, 農学部, 教授 (90390864)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ウシ / IVF胚 / 胚発育・組織分化 / 遺伝子発現 / 伸長期胚

Outline of Final Research Achievements

The low conception rate of bovine in vitro fertilization (IVF) embryos compared to in vivo embryos is a problem. If the expression of factors involved in embryonic failure and abnormal differentiation and growth of IVF embryos can be regulated by the addition of DKK1 and other factors, it will lead to overcoming these abnormalities in bovine IVF embryos. We investigated the effects of serum and DKK1 on the expression of factors associated with abnormal differentiation and growth in bovine IVF embryos and the effects of addition of growth factors on elongation stage development of bovine IVF embryos. The results showed that the addition of DKK1 (100 ng/ml) to IVC medium without serum normalized the expression of IGFBP-3 and OCT-4 in IVF embryos. In addition, the results revealed that the IVF embryos were abnormal growth in the elongation phase compared to Vivo embryos. However, these defects were not normalized by the addition of DKK1 (100 ng/ml) and CSF2 (100 ng/ml) to the IVC medium.

Free Research Field

発生工学, 家畜繁殖学

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、近年世界的に盛んに実施されているウシIVF胚を利用した子牛生産において、受胎率を向上し得るIVC培地の開発や生産されたIVF胚の選抜などIVF技術を用いたウシ産子の生産率の向上に資するものである。