Elucidation of the long-term effects of intracellular disturbances during mouse preimplantation embryos

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06443
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42030:Animal life science-related
• Research Institution: University of Yamanashi
• Principal Investigator: Kishigami Satoshi 山梨大学, 大学院総合研究部, 教授 (10291064)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 発生工学 / 着床前胚の体外培養 / 着床前発生 / 長期影響 / DOHaD

Outline of Final Research Achievements

In vitro culture technology of mammalian preimplantation embryos is essential for human infertility treatment and developmental engineering technology. In recent years, it has been suggested that the nutritional environment of the embryo not only affects the subsequent development rate and birth rate, but also the development of the fetus and placenta, as well as the risk of diseases such as lifestyle-related diseases in adults. The image and detailed mechanism remain unknown.
In this study, we clarified the effects of embryonic glucosamine pathway, endoplasmic reticulum stress, and culture in alphaMEM medium on embryos and offspring. In particular, malformation of the nucleolus, which is one of the organelles, was found in the culture of alphaMEM culture medium, which will develop diabetes in the future. suggested the possibility.

Free Research Field

発生工学分野

Academic Significance and Societal Importance of the Research Achievements

哺乳類の着床前胚の体外培養技術は、ヒトの不妊治療に加えて家畜および実験動物にも広く利用されている。しかし体内に比べて、体外培養により産子率が低下すること、産子や胎盤の発生に影響し、さらに成体においても糖代謝の異常など長期影響がみられるなど現在の体外培養技術の課題がある。本研究成果から、胚環境において糖などの栄養やストレスが発生率を低下させ、また核小体の攪乱を通じて長期影響のリスクに影響を及ぼす可能性が示唆された。今後これらの成果をもとに、発生低下および長期影響を克服した胚の発生に適した新規培養液の開発につながり、特にヒト不妊治療において大きく貢献することが期待される。