Analysis of H2A.Z forming transcriptionally active chromatin structures

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06496
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 43010:Molecular biology-related
• Research Institution: Japanese Foundation for Cancer Research
• Principal Investigator: Tachiwana Hiroaki 公益財団法人がん研究会, がん研究所 がん生物部, 研究員 (70546382)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ヒストンバリアントt / クロマチン

Outline of Final Research Achievements

In this study, we found a correlation between chromatin structure and histone incorporation. In the case of transcriptionally repressed, highly condensed chromatin, histone incorporation occurs only by a mechanism coupled to DNA replication. While in the case of transcriptionally active, less condensed chromatin, histone incorporation occurs in a DNA replication-independent manner. This indicates that the localization of each histone variant on genomic DNA is regulated by chromatin structure.

Free Research Field

エピジェネティクス

Academic Significance and Societal Importance of the Research Achievements

本研究成果はエピジェネティクス研究の重要な課題であるクロマチン構造による転写制御機構の解明をおこなった。本研究により、クロマチン構造による転写制御機構が明らかになり、遺伝子発現プロファイルが変化する生命現象（発生、分化、細胞のがん化、外部刺激への応答など）の理解につながることが期待される。