Investigating the physiological function and molecular mechanisms of cis-NATs responding to TGF_beta

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K06541
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 43030:Functional biochemistry-related
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Ohhata Tatsuya 浜松医科大学, 医学部, 助教 (80616459)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: TGFβ / cis-NATs / プログラム

Outline of Final Research Achievements

TGFβ is a growth factor with dual functions to promote or suppress tumor growth. cis-NATs are a class of antisense transcripts transcribed from the same genomic region of the sense strand. In this proposal, we attempted to comprehensively identify the TGFβ-responsive cis-NATs and elucidate their physiological functions and molecular mechanisms.
In this study, we developed an original program called CCIVR and successfully identified several functional TGFβ-responsive cis-NATs (Ohhata T et al., Sci Rep 2022). In addition, we developed another original program, CCIVR2, which also facilitates the identification of non-overlapping antisense transcripts (Suzuki M et al., Sci Rep., 2023). CCIVR/CCIVR2 is therefore a valuable bioinformatics tool for the analysis of functional antisense transcripts in different species and numerous biological processes.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

cis-NATsは、センス鎖と同領域から転写される逆方向の転写産物である。本研究では、RNA-seqデータから網羅的にcis-NATsを同定できる独自プログラムCCIVRを開発した（Ohhata T et al., Sci Rep., 2022）。さらに非オーバーラップ型のアンチセンスRNAも探索できるCCIVR2も開発した（Suzuki M et al., Sci Rep., 2023）。CCIVR/CCIVR2は異なる生物種や多くの生物学的プロセスの機能的アンチセンス転写産物の解析に寄与する。