Functional analyses of chondroitin sulfate chains in bone remodeling and skeletal muscle plasticity

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06551
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 43030:Functional biochemistry-related
• Research Institution: Kobe Pharmaceutical University
• Principal Investigator: Mikami Tadahisa 神戸薬科大学, 薬学部, 講師 (20330425)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: コンドロイチン硫酸 / 組織リモデリング / 骨粗鬆症 / サルコペニア / 筋サテライト細胞

Outline of Final Research Achievements

Chondroitin sulfate (CS) chains are representative sulfated glycosaminoglycan polysaccharides covalently attached to distinct protein cores to form proteoglycans (CSPGs). CSPGs are ubiquitous extra/pericellular matrix components that contribute to creating microenvironmental niches to support diverse cellular processes, including cell adhesion, proliferation, differentiation, and regeneration. These multiple actions of CSPGs are largely exerted through the CS moieties with structural heterogeneity.
In this study, we focused on functional roles of CS chains in bone remodeling and skeletal muscle plasticity. We found the potential sites of action for CS chains to inhibit osteoclastogenesis, and the structural and functional characteristics of age-related CS chains in skeletal muscles.

Free Research Field

生化学、分子生物学、糖鎖生物学

Academic Significance and Societal Importance of the Research Achievements

本研究結果から、細胞周囲の微細環境を形づくるCS鎖が、骨や骨格筋といった運動器を支える構成要素の分化・再生過程を調節する多機能糖鎖であることを裏付けることができた。CS鎖の質的・量的な発現制御に主眼を置いた本研究の成果は、運動器症候群の原因疾患である骨粗鬆症やサルコペニアに対する新たな治療戦略の基盤構築に大きく貢献するものと期待される。