2022 Fiscal Year Final Research Report
Spindle function and formation regulated by opt-controllable kinesins
| Project/Area Number |
20K06635
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44010:Cell biology-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
須河 光弘 東京大学, 大学院総合文化研究科, 助教 (80626383)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | モータータンパク質 / 分裂期キネシン / 光応答性タンパク質 |
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| Outline of Final Research Achievements |
For kinesin-6 functioning during anaphase, it was found, in an in vitro experiment, that single kinesin-6 molecules in dimeric forms were able to move processively on the microtubules under conditions of very low external load. We also found that kinesin-6 molecules move helically along suspended microtubules. On the other hand, in the presence of external load, their processivity were low. With a light-responsive protein for human kinesin-5 functioning during metaphase, we investigated the conditions regulating the binding and dissociation of kinesin-5 to microtubules with and without light irradiation at specific wavelengths.
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| Free Research Field |
細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
モータータンパク質キネシンは多種類存在し、各種キネシンは微小管上で力を発生し、細胞分裂等の物理的な現象に関わる酵素である。細胞分裂の分子機構を理解するには各種キネシンの力学的特性を明らかにしてゆくことも重要であり、本研究ではキネシン-6の力学特性の一端を明らかにした点で学術的意義がある。また、キネシンの力発生を従来法よりも迅速かつ局所的に制御可能な可視光照射で行う手法の開発を進めた。今後、キネシン機能の光制御法の確立によって細胞分裂中の各種キネシン機能が明らかになることが期待できる。
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