2022 Fiscal Year Final Research Report
Analysis of a novel exocytosis mechanoisms via lysosome in Dopaminergic neurons
Project/Area Number |
20K06646
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 44010:Cell biology-related
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Research Institution | Doshisha University |
Principal Investigator |
Torii Tomohiro 同志社大学, 脳科学研究科, 准教授 (00515603)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | ドーパミン作動性ニューロン / Arf6 / エキソサイトーシス / cytohesin-2 |
Outline of Final Research Achievements |
We previously have reported Arf6 activator cytohesin-2 interacted with a novel protein Coiled coil domain containing protein 120 (CCDC120) and the complex was transported along neuronal axons. Also we observed the complex localized in lysosome and endsomes of cells. We also observed large size vesicles existed in CCDC120 deletion mutant expressing cells. Taken together with these results, we suggest the complex including Arf6/cytohesin-2/CCDC120 might regulate fusion of vesicle and cellular transport. Also these molecular mechanisms might associate with molecular pathogenesis of Parkinson disease.
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Free Research Field |
細胞生物学、神経化学
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Academic Significance and Societal Importance of the Research Achievements |
パーキンソン病原因分子LRRK2は、国内外の多くの研究者の関心の的であるため、LRRK2の機能解析を報告している論文は多い。特にLRRK2は、Rab35(Bae et al., 2018)やRab10(Eguchi et al. 2018)などRabファミリー蛋白質を標的としたパーキンソン病の発症機構やストレス応答機構に関する論文は、それぞれ高く評価されている。これら分子機構にArf6が関与する可能性が非常に高いため、本研究の学術的意義うあ社会的意義が十分あると考えている。
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